siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Identifieur interne : 000D56 ( Pmc/Checkpoint ); précédent : 000D55; suivant : 000D57siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells
Auteurs : C.-Y. Lu ; H.-Y. Huang ; T.-H. Yang ; L.-Y. Chang ; C.-Y. Lee ; L.-M. HuangSource :
- European Journal of Clinical Microbiology & Infectious Diseases [ 0934-9723 ] ; 2008.
Abstract
The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.
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DOI: 10.1007/s10096-008-0495-5
PubMed: 18449585
PubMed Central: 7088151
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells</title><author><name sortKey="Lu, C Y" sort="Lu, C Y" uniqKey="Lu C" first="C.-Y." last="Lu">C.-Y. Lu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, H Y" sort="Huang, H Y" uniqKey="Huang H" first="H.-Y." last="Huang">H.-Y. Huang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Yang, T H" sort="Yang, T H" uniqKey="Yang T" first="T.-H." last="Yang">T.-H. Yang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Chang, L Y" sort="Chang, L Y" uniqKey="Chang L" first="L.-Y." last="Chang">L.-Y. Chang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lee, C Y" sort="Lee, C Y" uniqKey="Lee C" first="C.-Y." last="Lee">C.-Y. Lee</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, L M" sort="Huang, L M" uniqKey="Huang L" first="L.-M." last="Huang">L.-M. Huang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18449585</idno><idno type="pmc">7088151</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7088151</idno><idno type="RBID">PMC:7088151</idno><idno type="doi">10.1007/s10096-008-0495-5</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000373</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000373</idno><idno type="wicri:Area/Pmc/Curation">000373</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000373</idno><idno type="wicri:Area/Pmc/Checkpoint">000D56</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000D56</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells</title><author><name sortKey="Lu, C Y" sort="Lu, C Y" uniqKey="Lu C" first="C.-Y." last="Lu">C.-Y. Lu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, H Y" sort="Huang, H Y" uniqKey="Huang H" first="H.-Y." last="Huang">H.-Y. Huang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Yang, T H" sort="Yang, T H" uniqKey="Yang T" first="T.-H." last="Yang">T.-H. Yang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Chang, L Y" sort="Chang, L Y" uniqKey="Chang L" first="L.-Y." last="Chang">L.-Y. Chang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Lee, C Y" sort="Lee, C Y" uniqKey="Lee C" first="C.-Y." last="Lee">C.-Y. Lee</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Huang, L M" sort="Huang, L M" uniqKey="Huang L" first="L.-M." last="Huang">L.-M. Huang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">European Journal of Clinical Microbiology & Infectious Diseases</title><idno type="ISSN">0934-9723</idno><idno type="eISSN">1435-4373</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Groneberg, Da" uniqKey="Groneberg D">DA Groneberg</name></author><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name></author><author><name sortKey="Low, De" uniqKey="Low D">DE Low</name></author><author><name sortKey="Lode, H" uniqKey="Lode H">H Lode</name></author><author><name sortKey="Welte, T" uniqKey="Welte T">T Welte</name></author><author><name sortKey="Zabel, P" uniqKey="Zabel P">P Zabel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hsueh, Pr" uniqKey="Hsueh P">PR Hsueh</name></author><author><name sortKey="Hsiao, Ch" uniqKey="Hsiao C">CH Hsiao</name></author><author><name sortKey="Yeh, Sh" uniqKey="Yeh S">SH 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Eur J Clin Microbiol Infect Dis</journal-id><journal-id journal-id-type="iso-abbrev">Eur. J. Clin. Microbiol. Infect. Dis</journal-id><journal-title-group><journal-title>European Journal of Clinical Microbiology & Infectious Diseases</journal-title></journal-title-group><issn pub-type="ppub">0934-9723</issn><issn pub-type="epub">1435-4373</issn><publisher><publisher-name>Springer-Verlag</publisher-name><publisher-loc>Berlin/Heidelberg</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">18449585</article-id><article-id pub-id-type="pmc">7088151</article-id><article-id pub-id-type="publisher-id">495</article-id><article-id pub-id-type="doi">10.1007/s10096-008-0495-5</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>siRNA silencing of angiotensin-converting enzyme 2 reduced severe acute respiratory syndrome-associated coronavirus replications in Vero E6 cells</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Lu</surname><given-names>C.-Y.</given-names></name><address><phone>+886-2-23123456</phone><fax>+886-2-23934749</fax><email>cylu@ntuh.gov.tw</email></address><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>H.-Y.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>T.-H.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Chang</surname><given-names>L.-Y.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Lee</surname><given-names>C.-Y.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>L.-M.</given-names></name><xref ref-type="aff" rid="Aff1"></xref></contrib><aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.412094.a</institution-id><institution-id institution-id-type="ISNI">0000000405727815</institution-id><institution>Department of Pediatrics,</institution><institution>National Taiwan University Hospital,</institution></institution-wrap>No. 7, Chung-Shan South Road, Taipei, Taiwan</aff></contrib-group><pub-date pub-type="epub"><day>1</day><month>5</month><year>2008</year></pub-date><pub-date pub-type="ppub"><year>2008</year></pub-date><volume>27</volume><issue>8</issue><fpage>709</fpage><lpage>715</lpage><history><date date-type="received"><day>8</day><month>10</month><year>2007</year></date><date date-type="accepted"><day>15</day><month>2</month><year>2008</year></date></history><permissions><copyright-statement>© Springer-Verlag 2008</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>The outbreak of severe acute respiratory syndrome (SARS) in 2002–2003 has had a significant impact worldwide. No effective prophylaxis or treatment for SARS is available up to now. Angiotensin-converting enzyme 2 (ACE2) is the cellular receptor for SARS-associated coronavirus (SARS-CoV). By expressing a U6 promoter-driven small interfering RNA containing sequences homologous to part of ACE2 mRNA, we successfully silenced ACE2 expression in Vero E6 cells. By detecting negative strand SARS-CoV RNA and measuring RNA copy numbers of SARS-CoV by real-time reverse transcription polymerase chain reaction (RT-PCR), we demonstrated that SARS-CoV infection was reduced in the ACE2-silenced cell lines. These findings support the involvement of ACE2 in SARS-CoV infections and provide a basis for further studies on potential use of siRNA targeting ACE2 as a preventive or therapeutic strategy for SARS.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Reverse Transcription Polymerase Chain Reaction</kwd><kwd>Severe Acute Respiratory Syndrome</kwd><kwd>Severe Acute Respiratory Syndrome</kwd><kwd>Quantitative Reverse Transcription Polymerase Chain Reaction</kwd><kwd>Severe Acute Respiratory Syndrome Patient</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer-Verlag 2008</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Chang, L Y" sort="Chang, L Y" uniqKey="Chang L" first="L.-Y." last="Chang">L.-Y. Chang</name><name sortKey="Huang, H Y" sort="Huang, H Y" uniqKey="Huang H" first="H.-Y." last="Huang">H.-Y. Huang</name><name sortKey="Huang, L M" sort="Huang, L M" uniqKey="Huang L" first="L.-M." last="Huang">L.-M. Huang</name><name sortKey="Lee, C Y" sort="Lee, C Y" uniqKey="Lee C" first="C.-Y." last="Lee">C.-Y. Lee</name><name sortKey="Lu, C Y" sort="Lu, C Y" uniqKey="Lu C" first="C.-Y." last="Lu">C.-Y. Lu</name><name sortKey="Yang, T H" sort="Yang, T H" uniqKey="Yang T" first="T.-H." last="Yang">T.-H. Yang</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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